Table of Contents
Molecular Biology International
Volume 2014, Article ID 519290, 8 pages
http://dx.doi.org/10.1155/2014/519290
Research Article

A Synthetic Interaction between CDC20 and RAD4 in Saccharomyces cerevisiae upon UV Irradiation

1Department of Biology, Dominican College, 470 Western Highway, Orangeburg, NY 10962, USA
2Department of Cell Biology, Duke University, Durham, NC, USA
3Millsaps College Jackson, MS, USA
4ADAMS, Rome, GA, USA

Received 15 November 2013; Revised 12 January 2014; Accepted 13 January 2014; Published 23 February 2014

Academic Editor: Malayannan B. Subramaniam

Copyright © 2014 Bernadette Connors et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Regulation of DNA repair can be achieved through ubiquitin-mediated degradation of transiently induced proteins. In Saccharomyces cerevisiae, Rad4 is involved in damage recognition during nucleotide excision repair (NER) and, in conjunction with Rad23, recruits other proteins to the site of damage. We identified a synthetic interaction upon UV exposure between Rad4 and Cdc20, a protein that modulates the activity of the anaphase promoting complex (APC/C), a multisubunit E3 ubiquitin ligase complex. The moderately UV sensitive Δrad4 strain became highly sensitive when cdc20-1 was present, and was rescued by overexpression of CDC20. The double mutant is also deficient in elicting RNR3-lacZ transcription upon exposure to UV irradiation or 4-NQO compared with the Δrad4 single mutant. We demonstrate that the Δrad4/cdc20-1 double mutant is defective in double strand break repair by way of a plasmid end-joining assay, indicating that Rad4 acts to ensure that damaged DNA is repaired via a Cdc20-mediated mechanism. This study is the first to present evidence that Cdc20 may play a role in the degradation of proteins involved in nucleotide excision repair.