A Synthetic Interaction between CDC20 and RAD4 in Saccharomyces cerevisiae upon UV Irradiation
Figure 2
DNA damage-induced RNR3-lacZ expression is reduced in Δrad4/cdc20 mutants that have been (a) exposed to 4-NQO or (b) UV irradiated. Strains were transformed with pZZ2 (gift from S. Elledge), which contains the large subunit of the ribonucleotide reductase complex (RNR3) fused in-frame to lacZ. Cultures were exposed to UV or 4-NQO, incubated for 4 hours, and assayed for β-galactosidase activity. Fold induction was calculated as the ratio of β-gal activity of treated cultures cells to that of untreated cultures. In both treatments, Δrad4 strains were capable of evoking RNR3-lacZ expression, while Δrad4/cdc20-1 strains did not significantly affect this activity relative to wild-type (Y2454) or cdc20-1 strains.
