A Synthetic Interaction between CDC20 and RAD4 in Saccharomyces cerevisiae upon UV Irradiation
Figure 4
The cdc20-1 mutant allele contributes to diminished expression of Rad4-GFP upon UV irradiation. Strains harboring cdc20-1 show less fluorescence of Rad4-GFP. Cultures were grown overnight at 22°C and then UV irradiated using a Stratagene UV Stratalinker 1800. An outgrowth of four hours was then allowed. Three replicates were performed, and percentages were determined on a sample size of greater than 150 cells. All images were captured using a Zeiss Imager M1 AXIO paired with a Photometrics Coolsnap HQ2 camera.
