Expression of TPM1𝜿, a Novel Sarcomeric Isoform of the TPM1 Gene, in Mouse Heart and Skeletal Muscle

Figure 2

Expression analysis of TPM1α and TPM1κ RNA in mouse heart and skeletal muscle by RT-PCR. Plate (a) illustrates the ethidium bromide stain. Plate (b) illustrates hybridization with TPM1α probe. Plate (c) illustrates hybridization with TPM1κ probe. Lane 1: heart cDNA amplified with Mus TPM1α/κ-1 (+)/Mus UTR (−). Lane 2: heart cDNA amplified with Mus TPM1α/κ-1 (+)/Mus TPM1α/κ-2 (−). Lane 3: heart cDNA amplified with Mus TPM1κ-1 (+)/Mus TPM1α/κ-2 (−). Lane 4: heart cDNA amplified with TPM1α (+)/Mus TPM1α/κ-2 (−). Lane 5: heart cNDA amplified with Mus TPM1κ-1 (+)/Mus UTR (−). Lane 6: primer control for Mus TPM1α/κ-1 (+)/Mus UTR (−). Lane 7: primer control for Mus TPM1α/κ-1 (+)/Mus TPM1α/κ-2 (−). Lane 8: primer control for Mus TPM1κ-1 (+)/Mus TPM1α/κ-2 (−). Lane 9: skeletal cDNA amplified with Mus TPM1α/κ-1 (+)/Mus UTR (−). Lane 10: skeletal cDNA amplified with Mus TPM1α/κ-1 (+)/Mus TPM1α/κ-2 (−). Lane 11: skeletal cDNA amplified with Mus TPM1κ-1 (+)/Mus TPM1α/κ-2 (−). Lane 12: skeletal cDNA amplified with Mus TPM1α-1 (+)/Mus TPM1α/κ-2 (−).