Expression of TPM1, a Novel Sarcomeric Isoform of the TPM1 Gene, in Mouse Heart and Skeletal Muscle
Figure 4
Melt curves of SYBER green PCR and the agarose gel electrophoresis of PCR products with ethidium bromide staining. (a) Melt curves for amplified TPM1α and TPM1κ DNA. The peaks melting at one different temperature specific for TPM1α or TPM1κ demonstrate specific amplification of just one product for TPM1α and TPM1κ. The multiple curves represent the products from multiple replicates of the RT-PCR assay. (b) Agarose gel of PCR products with ethidium bromide staining to demonstrate amplification of a single product for TPM1α, TPM1κ, and 18S rRNA amplification. Lane 1 is heart, lane 2 is skeletal muscle, and lane 3 is for the respective primer control.
