Primer Based Approach for PCR Amplification of High GC Content Gene: Mycobacterium Gene as a Model

Figure 1

Prediction of secondary structure. (a) Presence of secondary structures in the primers designed by standard primer designing. Secondary structure halts the progression of polymerase. (b) Modification of bases at wobble position distorts secondary structure and allows moving of polymerase. Base modifications are shown in light red circle and the possible hydrogen bond is shown in square box.