Research Article
FOXO3a Gene Polymorphism Associated with Asthma in Indian Population
Table 2
Technical data for analysis of SNP in the human FOXO3a gene.
| | Gene name | SNP | Primers | GC content (%) | Annealing temperature (°C) | Restriction enzyme | PCR product size (bp) |
| | FOXO3a | rs13217795 | Forward: 5′-CTCCTTGGTCAGTTTGGTG-3′
Reverse: 5′-ATGAGTGAAGATGGAAGTAAGC-3′ | 52.6%
40.9% | 62°C | PagI | 667 bp |
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New England Biolabs Inc., USA.
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