Table of Contents
Molecular Biology International
Volume 2016, Article ID 1482796, 10 pages
http://dx.doi.org/10.1155/2016/1482796
Research Article

SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha

1Centre of Excellence in Biotechnology, M. P. Council of Science & Technology, Bhopal, Madhya Pradesh, India
2Bacteriology Laboratory, Department of Post Graduate Studies & Research in Biological Science, Rani Durgawati University, Jabalpur, Madhya Pradesh, India
3Madhya Pradesh Pollution Control Board, Bhopal, Madhya Pradesh, India

Received 31 October 2015; Revised 28 January 2016; Accepted 1 February 2016

Academic Editor: Sharad S. Singhal

Copyright © 2016 Pramod Kumar Sairkar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Commercially important Commiphora species are drought-tolerant plants and they are leafless for most of the year. Therefore, it is necessary to develop some molecular marker for the identification. Intended for that, in the present study, species-specific, sequence-characterized amplified regions (SCAR) markers were developed for proficient and precise identification of closely related species Commiphora wightii and C. myrrha, which may ensure the quality, safety, and efficacy of medicines made from these plants through adulterous mixing of these plants. Two species-specific RAPD amplicons were selected, gel-purified, cloned, and sequenced after screening of 20 RAPD primers. The sequence of 979 and 590 nucleotides (Genebank accession numbers K90051 and K90052) was used for development of 4 SCAR markers, namely, Sc1P, Sc1Pm, Sc2P, and Sc2Pm. Out of them, the Sc1Pm was specific for C. wightii, while Sc2P discriminated both the Commiphora species. These markers are first reported and will be useful for rapid identification of closely related Commiphora wightii and C. myrrha species.