Table of Contents
Molecular Biology International
Volume 2016, Article ID 1876065, 9 pages
http://dx.doi.org/10.1155/2016/1876065
Research Article

Molecular Characterization of Human Rotavirus from Children with Diarrhoeal Disease in Sokoto State, Nigeria

1Department of Veterinary Microbiology, Faculty of Veterinary Medicine, Usmanu Danfodiyo University, PMB 2346, Sokoto, Nigeria
2Department of Veterinary Microbiology, Usmanu Danfodiyo University, PMB 2346, Sokoto, Nigeria
3Department of Biochemistry, Faculty of Science, Usmanu Danfodiyo University, Sokoto, Nigeria
4Department of Veterinary Services, Ministry of Animal Health and Fisheries Development, Usman Faruk Secretariate, Sokoto, Nigeria

Received 7 October 2015; Revised 2 January 2016; Accepted 10 January 2016

Academic Editor: R. Holland Cheng

Copyright © 2016 B. R. Alkali et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was conducted to detect and characterize prevalent human group A rotavirus strains from 200 diarrheic children in Sokoto, Nigeria, by ELISA, monoclonal antibody (Mab) serotyping and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) techniques. Rotavirus was detected in 25.5% of the children. The G-serotypes observed in circulation were G4: 16 (59.3%), G1: 4 (14.8%), G2: 3 (11.1%), G3: 3 (11.1%), and G12: 1 (3.7%). The monoclonal antibody (Mab) serotyping detected G1 and G3 but did not detect G4 and G2 serotypes. The Mab typing of the G1 and G3 serotypes was consistent with the result of the RT-PCR. The VP4 genotypes detected were P 3 (13%), P 11 (47.8%), and the rare human P genotype (P), found in 9 patients (39.1%). Nine strains identified with the common G and P combinations were G4 P 5 (56%), G4 P 1 (11%), G1 P 2 (22%), and G3 P 1 (11%), while seven strains with unusual combinations or rare G or P genotypes identified were G12 P 1 (14%), G2 P 2 (29%), and G4 P 4 (57%). To our knowledge this is the first molecular study of human rotavirus and report of rare human G and P serotypes in Sokoto State.