Cloning, Sequencing, and the Expression of the Elusive Sarcomeric TPM4α Isoform in Humans

Table 1

Nucleotide sequences of the primer-pairs used for conventional and qRT-PCR analyses.

Primer	Isoform	Type of PCR	Sequence
TPM4-F-1 (P1+)	TPM4	Both conventional; & qRT-PCR	5′-CAGCCATGGAGGCCATCAAGA-3′
TPM4-R-1 (P2−)	TPM4	Conventional	5′-CACCATGTGAGAAGGACAGA-3′
TPM4-R-2	TPM4	qRT-PCR	5′-ACGAGCTGGATGCGTCGGT-3′
TPM4-F-1 (P3+)	TPM4	Conventional; & qRT-PCR	5′-ATGGCCGGCCTCAACTCCCTGG-3′
TPM4d-R-1	TPM4d	Conventional	5′-CACCATGTGAGAAGGACAGA-3′
TPM4d-R-2	TPM4d	qRT-PCR	5′-ACGAGCTGGATGCGTCGGT-3′
18s rRNA-F	18s rRNA	qRT-PCR	5′-TGCTGCAGTTAAAAAGCTCGTA-3′
18s rRNA-R	18s rRNA	qRT-PCR	5-ACCAACAAAATAGAACCGCGG-3′