Table of Contents
Molecular Biology International
Volume 2016, Article ID 4982131, 6 pages
http://dx.doi.org/10.1155/2016/4982131
Research Article

Total Protein Profile and Drug Resistance in Candida albicans Isolated from Clinical Samples

Biotechnology Pharmacology Laboratory, Centre for Scientific Research & Development, People’s University, Bhopal 462037, India

Received 17 March 2016; Revised 29 April 2016; Accepted 5 June 2016

Academic Editor: Sharad S. Singhal

Copyright © 2016 Kamal Uddin Zaidi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was done to assess the antifungal susceptibility of clinical isolates of Candida albicans and to evaluate its total protein profile based on morphological difference on drug resistance. Hundred and twenty clinical isolates of C. albicans from various clinical specimens were tested for susceptibility against four antifungal agents, namely, fluconazole, itraconazole, amphotericin B, and ketoconazole. A significant increase of drug resistance in clinical isolates of C. albicans was observed. The study showed 50% fluconazole and itraconazole resistance at 32 μg mL−1 with a MIC50 and MIC90 values at 34 and 47 and 36 and 49 μg mL−1, respectively. All isolates were sensitive to amphotericin B and ketoconazole. The SDS-PAGE protein profile showed a prevalent band of ~52.5 kDa, indicating overexpression of gene in 72% strains with fluconazole resistance. Since the opportunistic infections of Candida spp. are increasing along with drug resistance, the total protein profile will help in understanding the evolutionary changes in drug resistance and also to characterize them.