Drosophila Enhancer of Rudimentary Homolog, ERH, Is a Binding Partner of RPS3, RPL19, and DDIT4, Suggesting a Mechanism for the Nuclear Localization of ERH

Table 1

Primers used in the construction of e(r) fusion genes.

Species	Target	Primer sequences 5′ to 3′	Vector	Restriction site
H.s.	ERH 1 5′	TGGAATTCTCTCACACCATTTTG	pEG202	EcoRI
H.s.	ERH 52 5′	TGGAATTCGACATCAGTCAGTTGTTT	pEG202	EcoRI
H.s.	ERH 51 3′	TGCTCGAGTTAGATAGAGGGACT	pEG202	XhoI
H.s.	ERH 104 3'	GGCTCGAGTTACCCAGCCTGTT	pEG202	XhoI
D.m.	ERH 1 5′	GGGGATCCCCTCGCACACCATCCT	pEG202	BamHI
D.m.	ERH 25 5′	GGGGATCCCCAACGAGTGCATGGG	pEG202	BamHI
D.m.	ERH 52 5′	GGGGATCCCCGACATTAGCCAGCTATTC	pEG202	BamHI
D.m.	ERH 24 3′	CCCTCGAGTTACTCGTAGTCACAGTA	pEG202	XhoI
D.m.	ERH 51 3′	CCCTCGAGTTAAATGGTCGGAGTGTT	pEG202	XhoI
D.m.	ERH 104 3′	GGCTCGAGTTAATTGGAACTAA	pEG202	XhoI
H.s.	ERH 1 5′	AGATCTCGAGCTTCTCACACCATTTTGCTGG	pEGFP-C1	XhoI
H.s.	ERH 52 5′	TGCTCGAGCTGACATCAGTCAGTTGTTT	pEGFP-C1	XhoI
H.s.	ERH 51 3′	TGGGATCCTTAGATAGAGGGACT	pEGFP-C1	BamHI
H.s.	ERH 104 3′	GGTTTCGGATCCCGATTGAACAAGATCCTCAC	pEGFP-C1	BamHI
		e(r) sequence starts within the 3′ UTR.
D.m.	ERH 1 5′	GGCTCGAGCTTCGCACACCATCCT	pEGFP-C1	XhoI
D.m.	ERH 25 5′	GGCTCGAGCTAACGAGTGCATGGAGGG	pEGFP-C1	XhoI
D.m.	ERH 52 5′	GGCTCGAGCTGACATTAGCCAGCTATTC	pEGFP-C1	XhoI
D.m.	ERH 24 3′	CCGGATCCTTACTCGTAGTCACAGTA	pEGFP-C1	BamHI
D.m.	ERH 51 3′	CCGGATCCTTAAATGGTCGGAGTGTT	pEGFP-C1	BamHI
D.m.	ERH 104 3′	CCGGATCCTTAATTGGAACTAA	pEGFP-C1	BamHI

The target indicates the amino-acid codon that starts the e(r) sequence in the primer and the position of the primer. In the primer sequence, the restriction site is underlined and the starting codon is double underlined and in bold. For the 3′ primers, the stop codon is italicized. For the H.s. ERH 104 3′ for pEGFP-C1 the primer starts in the 3′ UTR, so there is not a stop codon in this primer. D.m., Drosophila melanogaster; H.s., Homo sapiens.