Drosophila Enhancer of Rudimentary Homolog, ERH, Is a Binding Partner of RPS3, RPL19, and DDIT4, Suggesting a Mechanism for the Nuclear Localization of ERH

Table 2

Drosophila ERH interactions with its partners.

ERH bait segment	Prey protein	Interaction
1–104	D.m. RPS3	+
1–104	H.s. RPS3	+
1–104	D.m. RPL19	+
1–51	D.m. RPS3	+
1–24	D.m. RPS3	+
1–24	D.m. RPL19	+
52–104	D.m. RPS3	−
25–51	D.m. RPS3	−

The table summarizes the bait-prey interactions performed in the yeast two-hybrid interaction assay. The amino acid segments of the ERH baits are given. A + interaction indicates that yeast containing a prey and the bait exhibits both the galactose-dependent growth on medium lacking leucine (LEU⁺) and the galactose-dependent beta-galactosidase activity (LACZ⁺) on medium containing X-gal. A − interaction indicates that the yeast failed both of the galactose-dependent assays. D.m., Drosophila melanogaster; H.s., Homo sapiens.