[¹⁸F]PARPi Imaging Is Not Affected by HPV Status In Vitro

Figure 1

PARP1, RAD51, and ɣH2AX expression in HPV-negative and HPV-positive cell lines. (a) Western blot analysis of three HPV-negative (FaDu, Cal 27, and SCC 15) and three HPV-positive (SCC 090, SCC 154, and SCC 152) cell lines, both with and without external beam irradiation (2 Gy). Vinculin and β-actin served as loading controls. For irradiated cell lines, protein extraction was performed 24 h postirradiation. For irradiated PARP1 and RAD51 detection, the same gel was used; therefore, the same loading control is considered. (b) Quantification of PARP1, RAD51, and ɣH2AX from (a). Bars represent grouped of 3 HPV-/+ cell lines.

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