[¹⁸F]PARPi Imaging Is Not Affected by HPV Status In Vitro

Figure 2

Effect of radiation on HPV-negative and HPV-positive cells. (a) Immunofluorescence staining using ɣH2AX and RAD51 antibodies performed on three HPV-negative (FaDu, Cal 27, and SCC 15) and three HPV-positive (SCC 090, SCC 154, and SCC 152), irradiated (2 Gy) and nonirradiated (0 Gy), cell lines. Representative images showing DAPI (blue), RAD51 (green), and ɣH2AX (red), 24 h postirradiation. IgG control in Supplementary Fig. S3. (b, c) Quantification of the mean number of ɣH2AX and RAD51 foci calculated per cell for different conditions (, Student -test).

(a)

(b)

(c)