Preclinical Evaluation of an Activity-Based Probe for Intraoperative Imaging of Esophageal Cancer

<div>VGT-309 labels esophageal cancer cell lines in a cathepsin activity-dependent manner. (a). Fluorescence microscopy of OE19 (human esophageal adenocarcinoma) cells at 100× magnification 1 hour after treatment with 1 <i>μ</i>M VGT-309. Cells were costained with DAPI and LysoTracker. VGT-309 labeling (shown in green) is predominantly lysosomal (shown in red) in distribution. Overlay images are shown at left with VGT-309 channel and LysoTracker channels shown at right. Scale bars represent 5 <i>μ</i>m. (b). Fluorescence microscopy at 10× and 40× (inset images) showing VGT-309 fluorescence in esophageal cancer cells with and without pretreatment with JPM-OEt, showing cathepsin activity dependence of fluorescence. Scale bars represent 20 <i>μ</i>m.</div>

Molecular Imaging

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Figure 3

Figure 3: Preclinical Evaluation of an Activity-Based Probe for Intraoperative Imaging of Esophageal Cancer 