13-cis-Retinoic Acid Affects Brain Perfusion and Function: In Vivo Study

Figure 3

Histological findings of brain tissues stained with hematoxylin-eosin (HE) in rats using light microscopy. (a) The normal (control group) rat brain showed a uniform and normal arrangement and structure of cerebral capillaries (capillary structure, Cap) and blood vessels with normal configuration. (b) The L group (low13-RA dose) showed an increase in glycolipids (yellow x sign), congestion of cerebral blood vessels (short wide small arrow), and focal hemorrhage (long arrow). The hippocampal neurons were reduced to ghost cells, with no nuclear staining and a barely visible cell outline plus softening and hemorrhage (chevron arrows). (c) The H group (high dose) showed necrosis of neurons (small stars), neuronophagia (small circles), and neuronal edema (small crescent shape). Subarachnoid hemorrhage was also seen.

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