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Malaria Research and Treatment
Volume 2010, Article ID 126085, 5 pages
Research Article

Review of Temephos Discriminating Concentration for Monitoring the Susceptibility of Anopheles labranchiae (Falleroni, 1926), Malaria Vector in Morocco

1Laboratoire d'Entomologie Médicale, Institut National d'Hygiène, 27 Avenue Ibn Batouta, Agdal, Rabat 10090, Morocco
2Service de lutte Antivectorielle, Direction de l'Epidémiologie et de Lutte contre les Maladies, Agdal, Rabat 10080, Morocco
3Institut de Recherche pour le Développement (IRD), UR016, Caractérisation et Contrôle des Populations de Vecteurs, Montpellier Cedex 5, France

Received 30 September 2010; Accepted 3 December 2010

Academic Editor: Abdoulaye Diabaté

Copyright © 2010 C. Faraj et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


In Morocco, the resistance monitoring of Anopheles labranchiae larvae to temephos is done using discriminating concentration of 0.125 mg, which is half of the WHO recommended dose for Anopheles. However, this dosage seemed to be too high to allow an early detection of the resistance and its revision was found necessary. The present study was carried out during May-June 2008 and 2009 in nine provinces from the north-west of the country. The aim was to determine the lethal concentrations LC100 of temephos for the most susceptible populations and to define the discriminating dosage as the double of this value. The bioassays were conducted according to WHO standard operating protocol to establish the dose-mortality relationship and deduct the LC50 and LC95. The results of this study indicated that the LC100 obtained on the most susceptible populations was close to 0.05 mg/l. Therefore, the temephos discriminating dosage for susceptibility monitoring of An. labranchiae larvae in Morocco was set to be 0.1 mg/l.