The Vector Population Monitoring Tool (VPMT): High-Throughput DNA-Based Diagnostics for the Monitoring of Mosquito Vector Populations
Table 1
Mosquito and Plasmodium specimens suitable for use as controls in VPMT assays that are available from the Malaria Research and Reference Reagent Resource Centre (MR4) http://www.mr4.org/.
Applicable TaqMan assay
MR4 reference No.
Species/genotype
Further details
Species ID
MRA-495
An. arabiensis
Genomic DNA from KGB (MRA-339)
Species ID
MRA-142
An. gambiae
Genomic DNA from An. gambiae, G3 strain
Species ID
MRA-761
An. quadriannulatus
from South Africa
Plasmodium infection
MRA-102G
Plasmodium falciparum
Genomic DNA from P. falciparum 3D7
Plasmodium infection
MRA-341G
Plasmodium vivax
Genomic DNA from P. vivax ONG
Kdr assay
MRA-762
An. gambiae/homozygous wild type
KISUMU strain, considered the insecticide susceptible standard
Kdr assay
MRA334
An. gambiae/homozygous for L1014S
RSP strain, expresses the L1014S kdr mechanism
Rdl assay
MRA-762
An. gambiae/homozygous wild type
KISUMU strain, considered the insecticide susceptible standard
Rdl assay
MRA-495
An. arabiensis/homozygous wild type
Genomic DNA from KGB (MRA-339)
Rdl assay
MRA-116
An. gambiae/homozygous for A296G
P+DLRC+R+ strain, dieldrin resistant due to Rdl mutation (Ala296Gly)
Rdl assay
MRA764
An. arabiensis/homozygous for A296S
SENN strain, dieldrin resistant due to Rdl mutation (Ala296Ser)
