Figure 5: Hemozoin quantification in histological sections of malaria infected-cured mice. Five microscopic fields (400×) were chosen for each organ section from P. berghei NK65 infected-cured mice. The images were then converted to a grayscale (8 bit) image using Image J. The amount of hemozoin (Hz) observed was expressed as a percentage of the total area of the image (in pixels). Over time the amount of Hz in the bone marrow (cross) appeared to be stable, whereas it decreased in liver tissue (red diamond) and it increased in the spleen (blue circle).