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Malaria Research and Treatment
Volume 2017, Article ID 3468276, 6 pages
https://doi.org/10.1155/2017/3468276
Research Article

Major Polymorphisms of Genes Involved in Homocysteine Metabolism in Malaria Patients in Ouagadougou, Burkina Faso

1Laboratoire de Biologie Moléculaires et de Génétique (LABIOGENE), UFR-SVT, Université de Ouaga I Professeur Joseph KI-ZERBO, Ouagadougou, Burkina Faso
2Département Substances Naturelles (DSN), Institut de Recherche en Sciences Appliquées et Technologies (IRSAT), Ouagadougou, Burkina Faso
3Department of Microbiology and Immunology, School of Medical Sciences, University of Cape Coast, Cape Coast, Ghana
4Ecole Supérieure des Techniques Biologiques et Alimentaires (ESTBA-UL), Université de Lomé, Lomé, Togo

Correspondence should be addressed to Simplice Damintoti Karou; moc.liamtoh@uorakecilpmis

Received 23 February 2017; Accepted 18 April 2017; Published 18 May 2017

Academic Editor: Donatella Taramelli

Copyright © 2017 Noé Yameogo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study analyzed the four main polymorphisms of the genes in homocysteine metabolism in malaria patients. Forty-two randomly selected subjects, diagnosed positive for Plasmodium falciparum, were included. The four genotypes were detected by real-time PCR using the MTHFR 677C>T, MTHFR 1298A>C, MTR 2756A>G, and MTRR 66A>G detection kit (Sacace Biotechnologies REF: T01002-96-S). The results revealed frequencies of 90% 677CC, 10% 677CT, and 00% 677TT for MTHFR C677T; 78.6% 1298AA, 19% 1298AC, and 2.4% 1298CC for MTHFR A1298C; 61.9% 2756AA, 33.3% 2756AG, and 4.8% 2756GG for MTR A2756G; and 50% of 66AA, 45% of 66AG, and 5% of 66GG for MTRR A66G. Correlations were found between A2756G MTR genotypes and parasitaemia (), MTRR A66G and hemoglobin genotypes (), and MTHFR A1298C and sex (). This study demonstrated for the first time an association between the A2756G MTR alleles and Plasmodium falciparum malaria in Burkina Faso and gave an overview of the genotypic distribution of the major SNPs influencing the metabolism of homocysteine.