Age-Induced Loss of Mossy Fibre Synapses on CA3 Thorns in the CA3 Stratum Lucidum
Figure 1
Boundaries of the CA3 and the right dorsal anterior hippocampus used for volumetric analysis. Top panel images show a Nissl stained section from the right dorsal anterior hippocampus (a, b) with the CA3 subfield (purple and cell layer in green) and the right dorsal hippocampi (blue/yellow outline) demarcated in distinct colouring shown in (b). EM analysis and sections were obtained from the CA3 subregion. Bottom panel shows a CA3 pyramidal neuron orientation as described by Spruston [19] and its distinct cellular layers. The CA3 receives three main inputs from (i) entorhinal cortex (EC) layer 3 forming the perforant pathway, (ii) recurrent collateral CA3 commissural fibres, and (iii) the giant mossy fibre boutons from the dentate gyrus. The area demarcated in red in the CA3 stratum lucidum (SL) was chosen for EM analysis. Abbreviation: stratum radiatum (SR), stratum oriens, stratum pyramidal layer (SP). Scale bar represents 540 μm in (a) and (b).
