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Neuroscience Journal
Volume 2013, Article ID 906731, 4 pages
http://dx.doi.org/10.1155/2013/906731
Research Article

Teratogenic Effect of Crude Ethanolic Root Bark and Leaf Extracts of Rauwolfia vomitoria (Apocynaceae) on Nissl Substances of Albino Wistar Rat Fetuses

1Department of Anatomy, Faculty of Basic Medical Sciences, University of Calabar, PMB 1115 Calabar, Nigeria
2Department of Zoology and Environmental Biology, Faculty of Natural and Applied Sciences, University of Calabar, PMB 1115 Calabar, Nigeria
3Department of Anatomy, Faculty of Basic Medical Sciences, University of Uyo, PMB 1017 Uyo, Nigeria
4Department of Anatomy, Faculty of Basic Medical Sciences, Anambra State University, Uli, Nigeria

Received 23 November 2012; Revised 27 February 2013; Accepted 27 February 2013

Academic Editor: Volney L. Sheen

Copyright © 2013 Mokutima A. Eluwa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Rauwolfia vomitoria is a plant used for the treatment of insanity. The possible adverse effects of crude ethanolic root bark and leaf extract of the plant on Nissl substances of albino Wistar rat fetuses were studied using 25 mature female Wistar rats. The animals were divided equally into 5 groups, labeled A, B, C, D, and E. Group A was the control, while groups B, C, D, and E were the experimental. The female rats were mated with the males overnight, and the sperm positive day was designated as day zero of pregnancy. Oral doses of 150 mg/kg and 250 mg/kg body weight of the root bark extract were administered to groups B and C animals, respectively, while groups D and E animals received 150 mg/kg and 250 mg/kg body weight of the leaf extract, respectively, from day 7 to 11 of gestation. On day 20 of gestation, the rats were sacrificed, the fetuses brains extracted, and the cerebral cortices excised and routinely processed for Nissl substances using Cresyl fast violet staining method. Results showed reduced staining intensity of Nissl substances in the treated groups, especially those that received the root extract. Thus, the herbs may have adverse effects on protein synthesis within the cerebral cortex.