Antinociceptive and Antioxidant Activities of Phytol In Vivo and In Vitro Models
OH-scavenging activity of phytol. Hydroxyl radical scavenging activity was quantified using 2-deoxyribose oxidative degradation in vitro, which produces malondialdehyde by condensation with 2-thiobarbituric acid (TBA). System is MDA production from 2-deoxyribose degradation with FeSO4 and H2O2 alone. Other groups denote MDA production by FeSO4 and H2O2 in the presence of different concentrations of phytol (0.9, 1.8, 3.6, 5.4, and 7.2 ng/mL). Values represent mean ± SEM , experiments in duplicate. P < 0.001 versus system; P < 0.001 versus 0.9 ng/mL; P < 0.001 versus 1.8 ng/mL; (ANOVA and t-Student-Neuman-Keuls test).