Insights from a Paradigm Shift: How the Poly(A)-Binding Protein Brings Translating mRNAs Full Circle
Table 1
The eIF4E interaction domain is conserved in eukaryotic eIF4G and 4E-binding proteinsa.
Species
Protein
eIF4E binding site sequence
Wheat
eIF4G
RKKY SRD FLL TFAH
Wheat
eIFiso4G
RVRY SRD QLL DLRK
Arabidopsis thaliana
eIF4G
EKKY SRD FLL KFAD
Arabidopsis thaliana
eIFiso4G1
RVKY TRE QLL ELKE
Arabidopsis thaliana
eIFiso4G2
RVRF SRE EIL QHRE
Homo sapiens
eIF4GI
KKRY DRE FLL GFQF
Homo sapiens
eIF4GII
KKQY DRE FLL GFQF
Consensus:
...Y .RE .LL ....
F DI
Saccharomyces cerevisiae
eIF4G1
KYTYGPTFLLQFKD
Saccharomyces cerevisiae
eIF4G2
KYTYGPTFLLQFKD
Saccharomyces cerevisiae
p20
MIKYTIDELFQLKP
Homo sapiens
4E-BP1
RIIYDRKFLMECRN
Homo sapiens
4E-BP2
RIIYDRKFLLDRRN
Homo sapiens
4E-BP3
RIIYDRKFLLECKN
aSequence of the eIF4E binding site in wheat, Arabidopsis thaliana, human, and yeast eIF4G is shown as is sequence of the eIF4E binding site in the 4E binding proteins from yeast (Sc p20) and human (Hs4E-BP). The consensus is derived from eIF4G proteins in higher eukaryotes.
