Research Article
Xenic Cultivation and Genotyping of Pathogenic Free-Living Amoeba from Public Water Supply Sources in Uganda
Table 4
Estimation of evolutionary divergence between sequences.
| | | | Percent identity | | |
| | | | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | | |
| | Divergence | 1 | | 53.2 | 53.6 | 53.4 | 52.8 | 53.8 | 53.8 | 51.4 | 52.1 | 56.5 | 34.5 | 36.0 | 31.5 | 52.2 | 25.6 | 53.3 | 25.4 | 53.7 | 25.2 | 25.1 | 24.8 | 24.3 | 25.0 | 25.2 | 30.5 | 29.9 | 30.5 | 30.9 | 1 | KU884884 | | 2 | 239.1 | | 97.3 | 95.6 | 96.0 | 94.4 | 94.4 | 53.3 | 55.4 | 54.9 | 32.9 | 34.6 | 30.7 | 55.4 | 26.4 | 52.5 | 23.9 | 91.5 | 23.8 | 23.5 | 25.6 | 24.2 | 24.6 | 24.6 | 29.3 | 28.9 | 29.9 | 31.0 | 2 | KU894799 | | 3 | 220.3 | 1.9 | | 95.7 | 95.0 | 94.0 | 94.5 | 52.3 | 54.4 | 55.0 | 32.8 | 34.8 | 30.6 | 54.5 | 26.6 | 52.5 | 23.8 | 91.7 | 23.6 | 23.4 | 25.5 | 24.0 | 24.3 | 24.7 | 29.1 | 28.9 | 29.8 | 31.1 | 3 | KU894800 | | 4 | 205.1 | 3.0 | 3.7 | | 94.1 | 93.4 | 95.6 | 52.8 | 55.2 | 55.0 | 32.3 | 34.6 | 30.6 | 55.0 | 26.4 | 52.5 | 24.2 | 89.8 | 23.8 | 23.5 | 25.5 | 24.0 | 24.8 | 25.2 | 29.4 | 29.0 | 30.1 | 31.1 | 4 | KU894801 | | 5 | 218.7 | 4.0 | 4.2 | 3.3 | | 94.0 | 94.4 | 52.9 | 55.0 | 54.4 | 33.3 | 34.2 | 30.3 | 55.0 | 26.6 | 52.6 | 23.6 | 89.7 | 23.6 | 23.4 | 25.6 | 23.9 | 24.4 | 24.8 | 29.1 | 28.9 | 29.9 | 31.1 | 5 | KU894802 | | 6 | 212.0 | 4.8 | 6.0 | 5.3 | 4.0 | | 94.0 | 52.1 | 54.0 | 55.8 | 34.2 | 35.6 | 31.8 | 54.0 | 27.8 | 53.8 | 24.7 | 88.7 | 24.3 | 24.0 | 26.4 | 25.0 | 25.0 | 25.5 | 29.8 | 29.4 | 30.5 | 31.7 | 6 | KU894803 | | 7 | 226.2 | 3.3 | 4.5 | 1.7 | 4.3 | 5.7 | | 50.9 | 53.0 | 55.4 | 33.2 | 35.3 | 31.5 | 53.0 | 27.5 | 53.6 | 25.1 | 89.0 | 24.6 | 24.3 | 26.3 | 24.8 | 25.4 | 25.8 | 29.9 | 29.8 | 30.7 | 32.6 | 7 | KU894804 | | 8 | 131.0 | 145.9 | 140.9 | 142.4 | 144.7 | 144.1 | 138.8 | | 79.5 | 63.2 | 35.7 | 37.0 | 32.9 | 80.7 | 27.7 | 54.6 | 28.3 | 56.2 | 27.7 | 27.7 | 25.6 | 25.6 | 25.6 | 26.4 | 30.2 | 29.9 | 30.5 | 31.0 | 8 | KU894805 | | 9 | 127.1 | 104.3 | 103.0 | 101.4 | 103.2 | 104.8 | 100.6 | 48.4 | | 65.9 | 45.5 | 42.0 | 36.2 | 96.0 | 43.0 | 66.6 | 32.2 | 58.3 | 32.3 | 32.2 | 41.6 | 38.1 | 31.5 | 32.2 | 35.6 | 35.3 | 36.4 | 35.2 | 9 | KU894806 | | 10 | 133.5 | 181.9 | 177.7 | 163.7 | 179.3 | 167.1 | 158.4 | 50.0 | 38.9 | | 47.4 | 53.7 | 49.1 | 67.0 | 41.5 | 66.6 | 38.9 | 58.1 | 38.1 | 38.3 | 39.5 | 39.5 | 47.9 | 48.6 | 47.1 | 47.7 | 48.2 | 48.5 | 10 | KU894807 | | 11 | 141.0 | 165.3 | 163.6 | 165.3 | 149.0 | 147.2 | 156.4 | 64.0 | 26.4 | 48.3 | | 65.2 | 59.6 | 47.8 | 67.7 | 65.1 | 55.3 | 31.3 | 54.5 | 54.5 | 65.4 | 58.5 | 54.9 | 55.6 | 58.8 | 59.1 | 59.2 | 58.9 | 11 | KU894808 | | 12 | 136.8 | 169.1 | 170.9 | 163.3 | 167.7 | 156.2 | 153.4 | 53.1 | 34.2 | 31.6 | 42.4 | | 83.6 | 43.2 | 69.5 | 58.3 | 68.5 | 33.3 | 68.9 | 68.9 | 68.9 | 67.5 | 73.3 | 74.2 | 92.8 | 89.8 | 86.3 | 84.6 | 12 | KU894809 | | 13 | 121.6 | 139.1 | 140.4 | 139.2 | 138.4 | 133.2 | 131.4 | 47.2 | 33.8 | 30.4 | 42.2 | 7.9 | | 37.4 | 70.1 | 53.4 | 69.7 | 29.1 | 68.3 | 68.3 | 68.1 | 66.4 | 74.4 | 75.2 | 84.7 | 86.0 | 86.4 | 95.8 | 13 | KU894810 | | 14 | 127.9 | 102.9 | 102.6 | 101.4 | 101.7 | 103.4 | 98.7 | 45.6 | 6.9 | 32.9 | 21.1 | 29.4 | 29.6 | | 45.2 | 68.7 | 32.8 | 58.4 | 32.5 | 32.3 | 43.6 | 36.8 | 32.3 | 33.2 | 36.5 | 36.2 | 36.9 | 36.0 | 14 | KU894811 | | 15 | 133.0 | 123.8 | 125.7 | 124.7 | 119.2 | 115.6 | 118.0 | 46.3 | 6.6 | 36.2 | 24.8 | 24.1 | 27.2 | 0.0 | | 64.8 | 74.2 | 24.7 | 72.8 | 72.8 | 96.4 | 82.7 | 73.0 | 73.2 | 68.6 | 68.6 | 69.4 | 70.5 | 15 | KU894812 | | 16 | 135.7 | 150.7 | 152.3 | 147.4 | 142.3 | 135.0 | 139.5 | 56.3 | 13.2 | 39.9 | 18.8 | 30.8 | 30.4 | 7.6 | 4.6 | | 49.3 | 50.6 | 47.2 | 47.2 | 61.7 | 54.2 | 48.6 | 49.4 | 51.5 | 51.7 | 52.9 | 52.9 | 16 | KU894813 | | 17 | 121.1 | 135.9 | 139.0 | 136.2 | 136.6 | 130.2 | 127.5 | 36.3 | 31.1 | 39.0 | 43.2 | 24.7 | 26.3 | 29.0 | 29.2 | 31.9 | | 22.0 | 95.2 | 95.2 | 71.5 | 70.1 | 69.9 | 70.3 | 68.2 | 68.1 | 69.1 | 71.1 | 17 | KU894814 | | 18 | 206.5 | 4.5 | 4.7 | 4.6 | 6.8 | 6.8 | 5.1 | 141.5 | 103.9 | 156.8 | 149.9 | 145.3 | 122.7 | 103.1 | 114.0 | 135.8 | 124.7 | | 23.2 | 23.0 | 25.0 | 23.5 | 24.2 | 24.6 | 29.0 | 28.7 | 29.7 | 29.4 | 18 | KM189419.1_Acanthamoeba_sp. | | 19 | 120.8 | 135.2 | 140.0 | 134.8 | 134.5 | 131.5 | 127.9 | 35.2 | 30.0 | 38.6 | 42.8 | 23.5 | 26.4 | 27.8 | 28.4 | 31.8 | 1.0 | 126.3 | | 99.5 | 75.6 | 74.0 | 74.0 | 74.1 | 72.2 | 71.9 | 72.8 | 69.0 | 19 | JQ271689.1_Hartmannella_vermiformis | | 20 | 122.2 | 139.9 | 144.9 | 139.4 | 139.0 | 135.6 | 131.8 | 35.2 | 30.5 | 38.1 | 42.8 | 23.5 | 26.4 | 28.3 | 28.4 | 31.8 | 1.0 | 130.3 | 0.6 | | 75.6 | 74.0 | 74.1 | 74.2 | 72.2 | 72.1 | 72.9 | 69.0 | 20 | KT185625.1_Vermamoeba_vermiformis | | 21 | 132.9 | 126.7 | 131.1 | 127.2 | 123.2 | 119.7 | 121.1 | 47.0 | 7.8 | 36.7 | 25.3 | 23.4 | 27.5 | 0.7 | 0.4 | 5.3 | 29.5 | 119.3 | 27.8 | 27.8 | | 85.5 | 76.1 | 76.0 | 71.8 | 71.8 | 72.3 | 68.5 | 21 | AF293895.1_Echinamoeba_exundans | | 22 | 131.8 | 130.7 | 135.2 | 131.1 | 130.7 | 123.1 | 124.2 | 46.2 | 14.5 | 36.4 | 35.8 | 25.2 | 29.0 | 16.1 | 14.9 | 17.1 | 31.5 | 123.0 | 29.9 | 29.9 | 14.9 | | 75.4 | 75.0 | 69.7 | 69.9 | 70.1 | 67.4 | 22 | AJ489261.1_Echinamoeba_thermarum | | 23 | 129.9 | 144.7 | 151.7 | 140.1 | 143.8 | 139.9 | 135.9 | 45.8 | 28.7 | 18.9 | 38.9 | 16.4 | 18.9 | 22.9 | 24.9 | 24.2 | 29.5 | 131.1 | 27.8 | 27.6 | 24.1 | 25.0 | | 97.0 | 76.4 | 78.0 | 77.3 | 75.0 | 23 | AY364635.1_Nuclearia_pattersoni | | 24 | 132.3 | 146.0 | 153.2 | 141.3 | 145.1 | 138.8 | 137.0 | 45.0 | 27.8 | 18.9 | 38.4 | 15.6 | 18.3 | 22.4 | 24.6 | 23.9 | 28.8 | 132.1 | 27.1 | 26.9 | 23.8 | 25.2 | 2.5 | | 76.9 | 78.5 | 78.0 | 75.8 | 24 | AF484687.1_Nuclearia_simplex | | 25 | 136.8 | 164.1 | 170.6 | 160.8 | 162.5 | 153.9 | 154.5 | 53.6 | 34.9 | 31.9 | 42.7 | 0.0 | 10.3 | 29.3 | 28.4 | 31.4 | 28.4 | 145.2 | 26.6 | 26.6 | 27.1 | 29.8 | 20.5 | 20.0 | | 96.1 | 91.7 | 85.5 | 25 | AF411276.1_Bodomorpha_minima | | 26 | 139.2 | 176.8 | 187.5 | 172.4 | 170.2 | 163.2 | 162.6 | 52.7 | 34.8 | 31.5 | 42.6 | 1.6 | 9.9 | 29.1 | 28.3 | 30.8 | 28.1 | 155.6 | 26.6 | 26.3 | 27.0 | 29.3 | 19.1 | 18.6 | 2.3 | | 93.6 | 86.7 | 26 | EU647174.1|_Cercomonadida | | 27 | 134.2 | 156.9 | 162.9 | 153.9 | 152.2 | 147.9 | 145.5 | 50.6 | 31.1 | 32.6 | 42.3 | 6.0 | 10.3 | 27.1 | 26.8 | 28.8 | 26.1 | 139.3 | 25.2 | 25.0 | 26.0 | 29.0 | 20.3 | 19.6 | 7.2 | 5.8 | | 87.2 | 27 | EU709140.1_Cercozoa_sp. | | 28 | 124.5 | 135.8 | 136.0 | 134.8 | 136.4 | 131.2 | 128.2 | 50.7 | 34.8 | 32.0 | 44.7 | 8.0 | 0.2 | 31.4 | 27.9 | 32.2 | 26.8 | 119.7 | 26.7 | 26.7 | 27.9 | 28.8 | 19.2 | 18.6 | 10.5 | 10.0 | 10.5 | | 28 | AY748806.1_Cercomonas_agilis |
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