Synthesis and Characterization of N¹-Phenylhydrazine-1,2-bis(carbothioamide) and Its Evaluation for Antimicrobial, Antioxidant, and Brine Shrimp Lethality Bioassay

Table 1

Determination of IC₅₀ values of the compound in DPPH.

Compound	Sl no.	Absorbance (Blank)	Concentration (μg/mL)	Absorbance	% Inhibition	IC50 (μg/mL)
Butylated hydroxytoluene (BHT)	1	0.378	500.000	0.025	94.709	16.46
	2	0.378	250.000	0.024	93.651
	3	0.378	125.000	0.035	90.741
	4	0.378	62.500	0.099	73.810
	5	0.378	31.250	0.149	60.582
	6	0.378	15.625	0.232	38.624
	7	0.378	7.813	0.264	30.159
	8	0.378	3.906	0.291	23.016
	9	0.378	1.953	0.303	19.841
	10	0.378	0.977	0.308	18.519
𝑁^1-Phenylhydrazine-1,2-bis (carbothioamide)	1	0.393	100.000	0.083	78.88	1.43
	2	0.393	50.000	0.084	78.62
	3	0.393	25.000	0.090	77.09
	4	0.393	12.500	0.096	75.57
	5	0.393	6.250	0.104	73.54
	6	0.393	3.125	0.111	71.75
	7	0.393	1.563	0.111	71.75
	8	0.393	0.781	0.226	42.49
	9	0.393	0.391	0.253	35.62
	10	0.393	0.185	0.368	6.36