Table 4: Expression levels of transcripts measured by real-time polymerase chain reaction (RT-PCR) from total RNA prepared from brown adipose tissues (BAT), skeletal muscles, kidney, subcutaneous fat (SQ fat), liver, and heart. The data are expressed as means ± SEM. values of UCN1 KO versus WT were calculated using Student’s -test. BAT: brown adipose tissue; UCP1: uncoupling protein 1; THR-α: thyroid hormone receptor-α subunit; THR-β: thyroid hormone receptor-β subunit; AdpR1: adiponectin receptor 1; AdpR2: adiponectin receptor 2; CRFR2: corticotropin-releasing hormone receptor 2.

GeneTissueSexGenotypeRQ( ) values

UCP1BATFemalesWT0.74 ± 0.3860.493
UCN1KO0.44 ± 0.238
MalesWT3.10 ± 1.5390.802
UCN1KO2.61 ± 1.119

THR-αSkeletal muscleFemalesWT2.22 ± 0.9360.731
UCN1KO1.74 ± 0.967
MalesWT2.40 ± 1.3080.959
UCN1KO2.49 ± 0.946

THR-βKidneyFemalesWT0.42 ± 0.1560.864
UCN1KO0.39 ± 0.118
MalesWT2.98 ± 0.8690.884
UCN1KO3.19 ± 1.167

AdiponectinSQ fatFemalesWT1.25 ± 0.2360.547
UCN1KO1.48 ± 0.286
MalesWT1.00 ± 0.3080.273
UCN1KO1.78 ± 0.647

AdpR1Skeletal muscleFemalesWT2.00 ± 0.2960.014
UCN1KO1.09 ± 0.168
MalesWT0.56 ± 0.1390.905
UCN1KO 0.60 ± 0.287

AdpR2LiverFemalesWT0.58 ± 0.1060.176
UCN1KO0.37 ± 0.108
MalesWT0.67 ± 0.1290.175
UCN1KO1.46 ± 0.549

CRFR2HeartFemalesWT0.28 ± 0.1560.368
UCN1KO0.96 ± 0.657
MalesWT4.04 ± 1.2180.319
UCN1KO6.14 ± 1.706