A Single Amino Acid Substitution in the Renal Betaine/GABA Transporter Prevents Trafficking to the Plasma Membrane
Figure 6
Confocal images of EGFP-tagged native BGT1 or T40A mutant expressed in MDCK cells following hypertonic stress for 5 h. At the end of this period, the cells were fixed and immunostained for furin (red fluorescence), a marker for the trans-Golgi network. BGT1 showed significant localization in the plasma membrane (panel (a)). In contrast, T40A remained intracellular and colocalized with furin (arrows, panels (b) and (c)). Cell outline is shown in white.
