Effects of the Demethylating Agent, 5-Azacytidine, on Expression of the Kallikrein-Kinin Genes in Carcinoma Cells of the Lung and Pleura
Figure 4
Effect of 5-azacytidine (5-AZA) on kallikrein-kinin mRNA expression in nonmalignant Met5A mesothelial cells and JU77, NO36, and LO68 malignant mesothelioma cells. (a) Fold changes in mRNA expression for tissue kallikrein (KLK1), plasma kallikrein (KLKB1), kinin B1 receptor (BDKRB1), kinin B2 receptor (BDKRB2), and high molecular weight kininogen (KNG1), relative to vehicle control (arbitrarily set at 1), in Met5A cells treated with 6.25 μM (white bars) or 25 μM (black bars) 5-AZA. (b) Fold changes in mRNA expression for KLK1, KLKB1, BDKRB1, and BDKRB2, relative to vehicle control (arbitrarily set at 1), in JU77 cells treated with 6.25 μM (white bars) or 25 μM (black bars) 5-AZA. (c) Fold changes in mRNA expression for KLK1, KLKB1, BDKRB1, BDKRB2, and KNG1, relative to vehicle control (arbitrarily set at 1), in NO36 cells treated with 6.25 μM (white bars) or 25 μM (black bars) 5-AZA. (d) Fold changes in mRNA expression for KLK1, KLKB1, BDKRB1, BDKRB2, and KNG1, relative to vehicle control (arbitrarily set at 1), in LO68 cells treated with 6.25 μM (white bars) or 25 μM (black bars) 5-AZA.
