Morphological Analysis of CDC2 and Glycogen Synthase Kinase 3β Phosphorylation as Markers of G2 → M Transition in Glioma
Figure 6
Elevated EGFR labeling correlates with increased p-GSKβ labeling. Tissue arrays for GBM (a–c), oligodendroglioma (d-e), and ependymoma (f) were stained with EGFR antibodies. Tissue cores showing membrane labeling for EGFR were quantified and pictures representative of these groups for the three tumors is presented. None of the ependymomas showed EGFR immunoreactivity. The number of cores positive and their corresponding percentages are placed in the bottom left of each panel. Correlations between pSer9GSK3β levels and phosphorylation state of CDC2 are plotted in (g). Elevated pSer9GSK3β levels are highly correlated with elevation of both activated and inhibited CDC2. Correlation between pSer9GSK3β levels and EGFR levels are plotted in (h). Most GBM cases show EGFR0020 expression correlated with GSK3β phosphorylation.
