Chronic Proliferative Dermatitis in Mice: NFκB Activation Autoinflammatory Disease
Figure 5
Increased keratinocyte apoptosis in the skin of Sharpincpdm mice. (a, b): Numerous apoptosis bodies in the epidermis and hair follicles of 6-week-old Sharpincpdm mice (a). No apparent apoptosis bodies were identified in the matched normal skin (b). (c, d): TUNEL immunofluorescence demonstrated that most apoptotic cells were located in the hair follicles, and some in the suprabasal epidermis, of 6-week-old Sharpincpdm mice (c). No TUNEL positive cells were found in the epidermis of normal skin (d). TUNEL positive cells also present in the dermis of both mutant and normal skin. (e, f): Annexin-V/propidium iodide FACS analysis confirmed apoptosis in the epidermis of 6-week-old mutants. 15.2% and 18.9% of total epidermal cells of the mutant mice were, respectively, late apoptotic or actually dead cells (e), compared to 1.07% and 7.67% of the wild-type epidermal cells (f).
