The Role of Molecular Pathology in the Diagnosis of Cutaneous Lymphomas

Figure 1

(a) IGH@ gene structure, VDJ rearrangement, and PCR primer annealing sites. The IGH@ gene is composed of ~45 V segments, ~23 D segments, and 6 J segments. DJ rearrangement occurs first, here combining D_H3 and J_H5. This is followed by V-DJ rearrangement, here combining V_H2 with the previously rearranged D_H3-J_H5. Multiple PCR primers are directed against the 3 FR regions in the V segment and a single primer to the J segment (FR4). N represents nucleotides inserted and deleted by TdT. Adapted with permission from [4]. (b) TRG@ gene structure, VJ rearrangement, and PCR primer annealing sites. The TRG@ gene is composed of 11 V segments and 5 J segments. VJ rearrangement here combines the V𝛾6 and J𝛾P2 segments. Multiple PCR primers are directed against V𝛾 segments and J𝛾 segments.

(a)

(b)