Immunoreactivity of the 14F7 Mab Raised against N-Glycolyl GM3 Ganglioside in Primary Lymphoid Tumors and Lymph Node Metastasis
Figure 1
Microphotographs of the 14F7 Mab reactivity in P3X63Ag.653 cell line after different fixation protocols. An intense, homogeneous, and finely granular reactivity of 14F7 Mab was observed in cells fixed with 4% neutral-buffered formaldehyde (a). Observe: both the slight diminishing in the intensity of reaction and the appearance of cells with a grosser granular staining mainly located in the cytoplasm after fixation with acetone (b) (black arrows). Inset on the upper-left corner, 400x magnification. The 14F7 Mab staining was only partially diminished after fixation with ethanol (c) while, the reactivity with this Mab was significantly removed in cells fixed and postfixed with methanol (d and e, resp.). No immunoreaction was detected in cells incubated with 1E10 Mab (negative control) (d). White bar = 50 μm.
