Table of Contents
Pathology Research International
Volume 2014, Article ID 308240, 7 pages
Research Article

Calretinin as a Diagnostic Adjunct for Ameloblastoma

1Department of Oral and Maxillofacial Pathology, Pacific Dental College and Hospital, PAHER University, Udaipur, Rajasthan 313024, India
2Department of Public Health Dentistry, Genesis Institute of Dental Sciences and Research, Ferozepur, Punjab 152001, India

Received 19 January 2014; Revised 27 March 2014; Accepted 28 March 2014; Published 15 April 2014

Academic Editor: Gary Tse

Copyright © 2014 Chitra Anandani et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Background. Calretinin is a 29 kDa calcium-binding protein of the EF-hand family which is expressed in a variety of normal and tumorigenic tissues. Its expression in odontogenic epithelium during odontogenesis and in neoplastic odontogenic tissues has been demonstrated. Unicystic ameloblastoma poses a diagnostic challenge, as its histologic presentation can be sometimes mistaken for keratocystic odontogenic tumor (KCOT). This study was performed to assess the usefulness of calretinin as a confirmatory marker for ameloblastic tissue. Methodology. Total of 40 cases: 16 unicystic ameloblastoma, 4 multicystic ameloblastoma, and 20 KCOT, were evaluated immunohistochemically for the presence, localization, distribution, and intensity of calretinin expression. Statistical analysis was done using Chi-square test to intercompare the expression between ameloblastoma and KCOT. Results. Sixteen cases of ameloblastoma (12 unicystic, 4 multicystic) showed positive calretinin staining of ameloblastic epithelium and only one case of KCOT was positive for calretinin, with the positivity restricted to the stellate reticulum like epithelium. Intercomparison between two groups revealed statistically significant difference . Conclusion. Calretinin appears to be a specific immunohistochemical marker for neoplastic ameloblastic epithelium and may be an important diagnostic adjunct in the differential diagnosis of ameloblastoma and KCOT.