High Expression of Galectin-3 in Patients with IgG4-Related Disease: A Proteomic Approach

Figure 1

Histologic features of IgG4-related disease (IgG4-RD) and liquid chromatography mass spectrometry (LS-MS) analysis. ((a) to (d)) Pancreatic tissue from patients with IgG4-related pancreatitis. (a) Fibrosis and aggregates of lymphocytes. Hematoxylin and eosin staining, scale bar, 100 μm. (b) Plasma cell infiltration as one of the histological features of IgG4-RD, Hematoxylin and eosins staining, scale bar, 20 μm. (c) Immunostaining with anti-IgG antibodies; or (d) with anti-IgG4 antibodies staining. IgG4/IgG > 90%, scale bar, 20 μm. IgG4 staining is one of the diagnostic criteria of IgG4-RD. (e) Volcano plot of all differential proteins showing a different abundance in IgG4-related pancreatitis () compared to the healthy pancreas () samples in our LC-MS analysis. Horizontal axis represents fold change compared to the respective protein in control samples. Vertical axis represents values. Proteins with a 2-fold change in their levels and a value lower that 0.05 were considered significant hits. Fold change values indicate higher (+) or lower (−) expression in IgG4-related pancreatitis samples compared with the controls. Significant proteins are labeled with their gene name in the figure.

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