Research Article
Lumbar Facet Joint Compressive Injury Induces Lasting Changes in Local Structure, Nociceptive Scores, and Inflammatory Mediators in a Novel Rat Model
| | Primer | Sequences | Tm | Gene No. |
| | TNF- | Forward: 5′-TCTGTGCCTCAGCCTCTTCTCATT-3′ | 60 | NM_012675.3 | | Reverse: 5′-TTGGGAACTTCTCCTCCTTGTTGG-3′ | | IL-1 | Forward: 5′-TCATCTTTGAAGAAGAGCCCGTCC-3′ | 60 | NM_031512.2 | | Reverse: 5′-TGCAGTGCAGCTGTCTAATGGGAA-3′ | | CGRP | Forward: 5′-TCTAGTGTCACTGCCCAGAAGAGA-3′ | 55 | NM_001033956.1 | | Reverse: 5′-GGCACAAAGTTGTCCTTCACCACA-3′ | | Substance P | Forward: 5′-TGGTCAGATCTCTCACAAAGG-3′ | 55 | NM_012666.2 | | Reverse: 5′-TGCATTGCGCTTCTTTCATA-3′ | | MMP-2 | Forward: 5′-ACCTCTTACAACAGCTGTACCACC-3′ | 60 | NM_031054.2 | | Reverse: 5′-TTTCCACCCACAGTGGACATAGCA-3′ | | BDNF | Forward: 5′-TCCTGGAGAAAGTCCCGGTATCAA-3′ | 60 | GQ395803.1 | | Reverse: 5′-TAGTTCGGCATTGCGAGTTCCAGT-3′ | | NK-1 | Forward: 5′-TGGGCAACGTAGTGGTGATA-3′ | 60 | NM_012667.2 | | Reverse: 5′-CACGGCTGTCATGGAGTAGA-3′ | | NK-2 | Forward: 5′-CCGAGCACCATTCTGTTTTT-3′ | 60 | NM_080768.1 | | Reverse: 5′-GGAGAGTCAACCGGTGTCAT-3′ | | Galanin | Forward: 5′-TTCCCACCACTGCTCAAGATG-3′ | 55 | NM_033237.1 | | Reverse: 5′-TGGCTGACAGGGTTGCAA-3′ | | Neuropeptide Y | Forward: 5′-AGATCCAGCCCTGAGACACTGATT-3′ | 55 | M15793.1 | | Reverse: 5′-TGGAAGGGTCTTCAAGCCTTGTTC-3′ | | -actin | Forward: 5′-TGTCACCAACTGGGACGATATGGA-3′ | 55 | NM_031144 | | Reverse: 5′-AGCACAGGGTGCTCCTCA-3′ |
|
|
