Potential for Cell-Transplant Therapy with Human Neuronal Precursors to Treat Neuropathic Pain in Models of PNS and CNS Injury: Comparison of hNT2.17 and hNT2.19 Cell Lines
Figure 4
Sensory behaviors after initiation of peripheral CCI pain and intrathecal transplant of parental hNT2 and hNT2.17 cells. For tactile allodynia after CCI and transplant of parental (a) hNT2 cells, adult female rats were either left unoperated, underwent CCI, or transplanted with nonviable hNT2 or viable hNT2 cells two weeks following CCI, one day following behavioral testing. Nonviable cells were prepared by suspension of the cells in water, centrifugation, and resuspension in buffer before transplant. All rats received 10 mg/Kg i.p. CsA at the time points corresponding to one day before and 13 days after cell transplant (daily injections). Animals were tested for hindpaw withdrawal to a graded series of von Frey hairs once every week for one week before and eight weeks following CCI and before and after transplants. Only animals that demonstrated tactile allodynia two weeks after CCI were transplanted. The data reported are the mean ± SEM of the difference scores for ligated paw minus the sham-operated paw of 14 animals in each group. The results with viable hNT2 cell transplants differed significantly from the CCI or nonviable graft conditions at each time point. . For thermal hyperalgesia after CCI and transplant of parental (b) hNT2 cells, animals were tested for hindpaw withdrawal once every week for one week before and eight weeks following CCI and before and after transplants. Only animals that demonstrated thermal hyperalgesia 2 weeks after CCI were transplanted. The data reported are the mean ± SEM of the difference values for ligated paw minus the sham-operated paw of 14 animals in each group. The viable hNT2 transplants differed significantly from the CCI and nonviable graft condition at each time point. . For tactile allodynia after CCI and transplant of GABA (c) hNT2.17 cells, adult female rats were either left unoperated, underwent CCI, or transplanted with nonviable hNT2.17 or viable hNT2.17 cells two weeks following CCI, one day following behavioral testing. Animals were tested for hindpaw withdrawal once every week for one week before and eight weeks following CCI and before and after transplants. Only animals that demonstrated tactile allodynia two weeks after CCI were transplanted. The data reported are the mean ± SEM of the difference scores for ligated paw minus the sham-operated paw of 14 animals in each group. The viable hNT2.17 cell transplants differed significantly from the CCI or nonviable graft conditions at each time point. . For thermal hyperalgesia after CCI and transplant of (d) hNT2.17 cells, animals were tested for hindpaw withdrawal once every week for one week before and eight weeks following CCI and before and after transplants. Only animals that demonstrated thermal hyperalgesia 2 weeks after CCI were transplanted. The data reported are the mean ± SEM of the difference values for ligated paw minus the sham-operated paw of 14 animals in each group. The viable hNT2.17 transplants differed significantly from the CCI and nonviable graft condition at each time point. .
