Growth Hormone-Releasing Peptide 6 Enhances the Healing Process and Improves the Esthetic Outcome of the Wounds

Table 1

Genes in study and PCR amplification data.

Symbol	Name	Gene Bank accession number		Sequence	Annealing temp. (°C)	Number of cycles	Product length (bp)
Rat oligonucleotides
Col1a1	Collagen, type I, alpha 1	NM_053304	Sense; antisense	CCCTCTGTGCCTCAGAAGAACT; GCCAGTCTGTTGGTCCATGTAG	58	30	234
Col3a1	Collagen, type III, alpha 1	NM_032085	Sense; antisense	AAGAGCGGAGAATACTGGGTTG; CAGGATTGCCATAGCTGAACTG	58	30	214
Tgfb1	Transforming growth factor, beta 1	NM_021578	Sense; antisense	TGCCAGAACCCCCATTGCTG; TCCACCTTGGGCTTGCGACC	70	35	700
Ctgf	Connective tissue growth factor	NM_022266	Sense; antisense	AGAGCTGGGTGTGTGTCCTCC; GCAGCAAACACTTCCTCGTGG	70	30	547
Acta2	Actin, alpha 2, smooth muscle, aorta	NM_031004	Sense; antisense	GTGCCTATCTATGAGGGCTATGCTCTGC; CATACTCCTGTTTGCTGATCCACATCTGC	68	35	601
Tnf	Tumor necrosis factor	NM_012675	Sense; antisense	ATGGCATGGATCTCAAAGACAA; TCTCCTGGTATGAAGTGGCAAA	58	40	150
Adam17	ADAM metallopeptidase domain 17	AJ012603	Sense; antisense	GTGACATGAATGGCAAATGTGA; TGGACAAGAATGCTGAAAGGAA	55	40	172
Cd36	CD36 molecule (thrombospondin receptor)	NM_031561	Sense; antisense	GTCGTATGGTGTGCTGGACATT; TGGCTTGACCAGTATGTTGACC	62	35	217
Vim	Vimentin	NM_031140	Sense; antisense	GGATTTCTCTGCCTCTTCCAAA; GTCATCGTGGTGCTGAGAAGTC	62	35	164
Des	Desmin	NM_022531	Sense; antisense	TCCGTGCTCAGTATGAGACCAT; GCATCAATCTCGCAGGTGTAGG	62	35	181
Pdgfb	Platelet-derived growth factor beta polypeptide	NM_031524	Sense; antisense	AACATGACCCGAGCACATTCT; TGGCTTCTTTCTCACAATTTCG	62	35	303
Fn1	Fibronectin 1	NM_019143	Sense; antisense	GTGGTCATTTCAGATGCGATTC; GGCTCCGAGATACTCTTTCTGC	62	35	227
B2m	Beta-2 microglobulin	NM_12512	Sense; antisense	CGGTGACCGTGATCTTTCTGGT; GGTGACGGTTTTGGGCTCCTT	58	30	332
Rabbit oligonucleotides
TGFB1	Transforming growth factor, beta 1	XM_008249704	Sense; antisense	TCATTTACCGTCACCTGGATTG; TGTGTAGATGTTGAGCCCGTTC	72	40	229
PDGFB	Platelet-derived growth factor beta polypeptide	XM_008257019	Sense; antisense	GGTGAGAAAGATCGAGATTGTGC; GTGTGCTTGAACTTGTGGTGCT	62	40	231
CTGF	Connective tissue growth factor	XM_008263527	Sense; antisense	GGGCTAAGTTCTGCGGAGTATG; CATTGTCCCCAGGACAGTTGTA	62	30	162
MMP3	Matrix metallopeptidase 3 (stromelysin 1, progelatinase)	NM_001082280	Sense; antisense	TCTCTTCCTTCAGCAGTGGATG; TTCCTTATCAGAAATGGCAGCA	58	40	186
COL1A1	Collagen, type I, alpha 1	XM_008271783	Sense; antisense	CCTGGGCAGAGAGGAGAAAGAG; CCTCACGTCCAGATTCACCAG	62	30	157
IGFBP3	Insulin-like growth factor binding protein 3	NM_000598 (human)	Sense; antisense	TGCCGTAGAGAAATGGAAGACA; GCCCATACTTATCCACACACCA	72	40	172
PPARG	Peroxisome proliferator-activated receptor gamma 1	AY166780	Sense; antisense	TGATGAATAAAGACGGGGTCCT; CCACTGAGAATGATGACGGCTA	62	40	187
P4HB	Prolyl 4-hydroxylase, beta polypeptide	NM_001171047	Sense; antisense	ATGACCAAGTACAAGCCCGAGT; GCGTAGAACTCCACGAAGACG	62	30	212
COL3A1	Collagen, type III, alpha 1	XM_002712333	Sense; antisense	AAAGAAAGCCCTGAAGCTGATG; CCACCAATATCATAGGGTGCAA	62	30	195
B2M	Beta-2 microglobulin	XM_002717921	Sense; antisense	CGCCCCAGATTGATATTGAGTT; GATCCCATTTCACTGTCATAGGC	62	30	195