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Sarcoma
Volume 3, Issue 2, Pages 107-113
http://dx.doi.org/10.1080/13577149977730

Expression of Smooth Muscle Calponin in Synovial Sarcoma

1Department of Orthopaedic Surgery, Osaka Medical Center for Cancer and Cardiovascular Diseases, 1-3-3, Nakamichi, Higashinari-ku, Osaka 537-8511, Japan
2Department of Medicine, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
3Department of Pathology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
4Department of Orthopaedic Surgery, Osaka University Medical School, Suita, Japan
5Department of Pathology, Kochi Medical School, Kochi, Japan
6Department of Gastrointestinal Oncology, Osaka Medical Center for Cancer and Cardiovascular Diseases, Osaka, Japan
7Graduate School of Pharmaceutical Science, Osaka University, Suita, Japan
8PRESTO, Japan Science and Technology Corporation (JST), Japan

Copyright © 1999 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. Histogenesis of synovial sarcoma remains controversial and reliable molecular markers for diagnosis are necessary. Expression of basic calponin, a smooth muscle differentiation-specific actin-binding protein, was studied in synovial sarcoma.

Subjects and Methods. The basic calponin gene and the gene product were analyzed by reverse transcription PCR analysis (RT-PCR) and immunohistochemistry in 14 synovial sarcomas and a human synovial sarcoma cell line (HS-SY-II).

Results and Discussion. Immunoreactivity for basic calponin was detected in the cytoplasm of 6 synovial sarcomas (43% positive). In the basic calponin-positive tumors and the HS-SY-II cells, expression for smooth muscle-specific genes, including basic calponin and SM22α , was detected by RT-PCR, suggesting a lineage relationship between synovial sarcoma cells and smooth muscle-like mesenchymal cells.

Conclusions. A subset of synovial sarcomas expressing the basic calponin gene and the gene product were identified. The basic calponin may have potential utility as a novel molecular marker identifying certain synovial sarcomas.