Immunohistochemical Estimates of Angiogenesis, Proliferative Activity, p53 Expression, and Multiple Drug Resistance Have No Prognostic Impact in Osteosarcoma: A Comparative Clinicopathological Investigation

Figure 1

Field of vision showing an osteosarcoma stained immunohistochemically by MIB-1 to demonstrate cells in proliferation cycle (brown nuclear stain). A counting frame has been superimposed onto the histologic section for estimation of the rate and index of neoplastic proliferation. Using an unbiased two-dimensional counting rule, nuclei in focus inside the frame or on the hatched, blue edges are counted, as long as they do not intersect with the fully drawn, red exclusion edges of the frame or their extensions. In this example, 6 nuclei in cycle (brown) and 10 “resting” nuclei (blue) are counted (original magnification: 400X).