Immunohistochemical Estimates of Angiogenesis, Proliferative Activity, p53 Expression, and Multiple Drug Resistance Have No Prognostic Impact in Osteosarcoma: A Comparative Clinicopathological Investigation
Figure 2
(a) Low-power view of a histologic section from an
osteosarcoma, showing a microvascular hot spot in the lower left corner (original
magnification: 40X). (b) Histologic section with the Chalkley graticule
superimposed. The graticule, equipped with 25 stochastically spaced points, is
rotated until the highest number of these 25 points coincides with immunohistochemically
stained microvascular profiles in the tumor tissue. The mean of three hot spot
counts is reported as the angiogenetic Chalkley number (immunohistochemical
stain by CD34 to highlight endothelial cells and vascular profiles; original
magnification: 200X).
