Synergism of Heat Shock Protein 90 and Histone Deacetylase Inhibitors in Synovial Sarcoma
Figure 4
Transcriptional activation of NF-B luciferase reporter. NF-B induction following treatment with (a) MS-275, (b) 17-AAG, and (c) Combination. SYO-1 cells were grown as monolayer cultures in 24 well plates and transfected with 0.3 g of NF-B luciferase reporter plasmid. Cells were
treated the following day for 24 hours and lysed with passive lysis buffer. Samples were aliquoted to plates and simultaneously assayed for luminosity by injection with 50 l/well of LARII reagent and protein quantity by copper sulfate/bichionic acid assay. Readings for luminosity were normalized to protein concentration and vehicle control was set to 1.00. Error bars represent 95% confidence intervals for three replicate measurements.
