Synergism of Heat Shock Protein 90 and Histone Deacetylase Inhibitors in Synovial Sarcoma
Figure 5
HDAC3 siRNA knockdown recapitulates the effects HDAC inhibitors on synovial sarcoma cells. (a) 50 nM HDAC3 siRNA promotes extensive cell death at 24 hours, as shown by staining of a large inviable cell fraction by 500 ng/mL propidium iodide, where virtually all cells exposed to equimolar scrambled siRNA control are viable. (b) Densitometric quantitation of Western blots for nuclear RelA, expressed relative to control siRNA transfected cells treated with vehicle (1.0).
