Therapeutic Antibodies Targeting CSF1 Impede Macrophage Recruitment in a Xenograft Model of Tenosynovial Giant Cell Tumor

Figure 1

Renal subcapsular implant model of tensosynovial giant cell tumor. (a) Fluorescence in situ hybridization on xenograft using red and green probes flanking the CSF1 locus, showing split probes in the rightmost cell, consistent with translocation of CSF1 sequences (objective magnification $100\text{x}$). ((b)-(c)) H&E histology of the xenograft: (b) host kidney on the left and tumor on the right (objective magnification $10\text{x}$); (c) centre of the xenograft (objective magnification $40\text{x}$). (d) Immunohistochemistry for CSF1 in xenograft tissue (objective magnification $20\text{x}$). ((e)-(f)) F4/80 immunohistochemistry ($20\text{x}$ objective magnification), showing host macrophage infiltration in PVNS xenografts: (e) PBS control, (f) mice treated with 5H4 anti-CSF1 antibody.

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