miRNA Profiling: How to Bypass the Current Difficulties in the Diagnosis and Treatment of Sarcomas
Figure 3
mTOR inhibition by RAD-001 in chondrosarcoma resulted in cell proliferation inhibition. (a) Cell growth was measured by the Cell Titer GloLuminescent assay as described in Section 2.6 24, 48, and 72 h after exposure to 100 nM RAD-001. Results were represented as the mean % of proliferation normalized to untreated cells of two independent experiments realized in duplicate. A Fisher test was realized, NS corresponds to “nonsignificant”. (b) Western blot analysis of RAD-001 effects on the mTOR pathway in the chondrosarcoma and 15-47 osteosarcoma cells exposed or not (NT) to doxorubicin (dox). 30 μg protein extracts were analysed by Western blot with antibodies 1/1000 against actors of the mTOR pathway (phosphorylated form or not) (Cell signalling, Beverly MA).
