Figure 3: Chondrosarcoma cells expression of maspin and 14-3-3σ proteins. Western blot analysis for maspin and 14-3-3σ. Lane 1 is the SNM83 chondrocyte cell line which shows no protein expression for either maspin or 14-3-3σ, and in lane 2 the NH69 chondrosarcoma cell line shows a robust induction of both proteins. These are consistent with real-time PCR results of mRNA expression. Beta-actin was used as the loading control.