Figure 4: Normal chondrocyte cells have a highly methylated maspin promoter and 14-3-3σ CpG island. (a) and (c) Histograms representing the percent methylation at each of the CpG’s spanning the maspin promoter and 14-3-3σ CpG island in SNM83 chondrocyte cells. (b) and (d) Bubble charts of Maspin and 14-3-3σ in SNM83. Each of the five rows represents a sequenced amplicon, while each of the columns represents the position of the nucleotide relative to the transcription start site. Darkened circles represent methylated cytosines while open circles represent unmethylated cytosines. Nucleotide positions relative to start site were based on UCSC genome browser build 17.