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Volume 2011, Article ID 691608, 8 pages
Research Article

A “Proteoglycan Targeting Strategy” for the Scintigraphic Imaging and Monitoring of the Swarm Rat Chondrosarcoma Orthotopic Model

1INSERM (UMR 990) Université d'Auvergne, rue Montalembert, BP 184, 63005 Clermont-Ferrand Cédex, France
2INSERM (UMR S957), Université de Nantes, 44035 Nantes, France
3Service d'Anatomo-Pathologie, CLCC Jean Perrin, 63001 Clermont-Ferrand, France
4Cyclopharma Laboratoires, 63360 Saint-Beauzire, France

Received 1 October 2010; Revised 24 December 2010; Accepted 4 January 2011

Academic Editor: Irene Andrulis

Copyright © 2011 Caroline Peyrode et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Our lab developed 99mTc-NTP 15-5 radiotracer as targeting proteoglycans (PGs) for the scintigraphic imaging of joint. This paper reports preclinical results of 99mTc-NTP 15-5 imaging of an orthotopic model of Swarm rat chondrosarcoma (SRC). 99mTc-NTP 15-5 imaging of SRC-bearing and sham-operated animals was performed and quantified at regular intervals after surgery and compared to bone scintigraphy and tumoural volume. Tumours were characterized by histology and PG assay. SRC exhibited a significant 99mTc-NTP 15-5 uptake at very early stage after implant (with tumour/muscle ratio of 1.61 ± 0.14), whereas no measurable tumour was evidenced. As tumour grew, mean tumour/muscle ratio was increased by 2.4, between the early and late stage of pathology. Bone scintigraphy failed to image chondrosarcoma, even at the later stage of study. 99mTc-NTP 15-5 imaging provided a suitable set of quantitative criteria for the in vivo characterization of chondrosarcoma behaviour in bone environment, useful for achieving a greater understanding of the pathology.