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Sarcoma
Volume 2011, Article ID 732690, 6 pages
http://dx.doi.org/10.1155/2011/732690
Research Article

Identification of Differentially Expressed MicroRNAs in Osteosarcoma

1Division of Hematology, Oncology and Stem Cell Transplantation, Children's Memorial Hospital, 2300 Children's Plaza, Chicago, IL 60614, USA
2Department of Pediatrics, Feinberg School of Medicine Northwestern University, Chicago, IL 60611, USA
3Cancer Biology and Epigenomics Program, Children's Memorial Research Center, Chicago, IL 60614, USA
4Division of Anatomic Pathology, Children's Memorial Hospital, Chicago, IL 60614, USA

Received 1 April 2011; Accepted 10 June 2011

Academic Editor: Alessandro Gronchi

Copyright © 2011 Rishi R. Lulla et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A limited number of reports have investigated the role of microRNAs in osteosarcoma. In this study, we performed miRNA expression profiling of osteosarcoma cell lines, tumor samples, and normal human osteoblasts. Twenty-two differentially expressed microRNAs were identified using high throughput real-time PCR analysis, and 4 (miR-135b, miR-150, miR-542-5p, and miR-652) were confirmed and validated in a different group of tumors. Both miR-135b and miR-150 have been previously shown to be important in cancer. We hypothesize that dysregulation of differentially expressed microRNAs may contribute to tumorigenesis. They might also represent molecular biomarkers or targets for drug development in osteosarcoma.